We are thrilled to share a new impactful scientific publication that contributes significantly to the field of immune profiling and translational immunology.
The article, titled “Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease”, published in the Journal of Immunological Methods, introduces SCRIPT, a novel stepwise deep immunophenotyping pipeline that integrates high-dimensional cytometry and single-cell RNA sequencing (scRNA-seq) to comprehensively characterize immune cell composition, functionality and molecular profiles at single-cell resolution. Among the authors is Dr. Feng Q. HeFeng from the Luxembourg Institute of Health, a valued member of IMMUPARKNET, who recently attended the Annual Conference in Coimbra with a presentation entitled “Immune Aging and its gender-specific link to Parkinson’s Disease”.
IMMUPARKNET is officially acknowledged in the funding section for supporting this research, reflecting the Action’s commitment to fostering high-quality methodological approaches and collaborative research in translational immunology.
Key highlights of the study:
🔹 Comprehensive immune profiling: the SCRIPT pipeline combines mass cytometry, focused flow cytometry and single-cell RNA sequencing to provide a high-dimensional characterization of immune cell composition, functionality and genome-scale molecular profiles.
🔹 Guided single-cell analysis: by using cytometry as a first screening step, the pipeline efficiently identifies the most relevant immune cell subsets for downstream scRNA-seq, reducing experimental costs and avoiding trial-and-error approaches.
🔹 Methodological guidance: the study provides practical recommendations covering pre-analytical procedures, quality control, sample processing and experimental design, supporting robust, reproducible and high-quality immune profiling across different research settings.
This work perfectly aligns with IMMUPARKNET’s mission to advance immune profiling strategies and promote methodological excellence to accelerate discoveries in Parkinson’s disease and other immune-mediated conditions.
Read the full open-access article here: ScienceDirect Link
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